Persönlicher Status und Werkzeuge

Sprachwahl

Selected Papers

Proteomics

Wilkins MR, Appel RD, Van Eyk JE, Chung MC, Görg A, Hecker M, Huber LA, Langen H, Link AJ, Paik YK, Patterson SD, Pennington SR, Rabilloud T, Simpson RJ, Weiss W, Dunn MJ.
Guidelines for the next 10 years of proteomics.
Proteomics. 2006 Jan;6(1):4-8.

IPG-Dalt Methodology

Görg A, Drews O, Lück C, Weiland F, Weiss W.
2-DE with IPGs.
Electrophoresis. 2009 Jun;30 Suppl 1:S122-32.

Görg A, Weiss W, Dunn MJ.
Current two-dimensional electrophoresis technology for proteomics.
Proteomics. 2004 Dec;4(12):3665-85. Review.

Görg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W.
The current state of two-dimensional electrophoresis with immobilized pH gradients.
Electrophoresis. 2000 Apr;21(6):1037-53.

Görg, A.
Two-dimensional electrophoresis.
Nature 1991 349:545-46

Görg A, Postel W, Gunther S.
The current state of two-dimensional electrophoresis with immobilized pH gradients.
Electrophoresis. 1988 Sep;9(9):531-46.

Görg, A., W. Postel, J. Weser, S. Günther, J.R. Strahler, S.M. Hanash and L. Somerlot:
Elimination of point streaking on silver stained two-dimensional gels by addition of
iodoacetamide to the equilibration buffer
Electrophoresis 1987, 8: 122-124.

Bjellqvist B, Ek K, Righetti PG, Gianazza E, Görg A, Westermeier R, Postel W.
Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications.
J Biochem Biophys Methods. 1982 Sep;6(4):317-39.

Narrow overlapping IPGs

Wildgruber R, Harder A, Obermaier C, Boguth G, Weiss W, Fey SJ, Larsen PM, Görg A.
Towards higher resolution: two-dimensional electrophoresis of Saccharomyces cerevisiae proteins using overlapping narrow immobilized pH gradients.
Electrophoresis. 2000 Jul;21(13):2610-6.

Alkaline IPGs

Drews O, Reil G, Parlar H, Görg A.
Setting up standards and a reference map for the alkaline proteome of the Gram-positive bacterium Lactococcus lactis.
Proteomics. 2004 May;4(5):1293-304.

Wildgruber R, Reil G, Drews O, Parlar H, Görg A.
Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Proteomics. 2002 Jun;2(6):727-32.

Görg A.
IPG-Dalt of very alkaline proteins.
Methods Mol Biol. 1999;112:197-209.

Görg A, Obermaier C, Boguth G, Weiss W.
Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: wide pH gradients up to pH 12, longer separation distances and simplified procedures.
Electrophoresis. 1999 Apr-May;20(4-5):712-7.

Görg A, Boguth G, Obermaier C, Weiss W.
Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient.
Electrophoresis. 1998 Jun;19(8-9):1516-9.

Görg A, Obermaier C, Boguth G, Csordas A, Diaz JJ, Madjar JJ.
Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins.
Electrophoresis. 1997 Mar-Apr;18(3-4):328-37.

Righetti PG, Bossi A, Görg A, Obermaier C, Boguth G.
Steady-state two-dimensional maps of very alkaline proteins in an immobilized pH 10-12 gradient, as exemplified by histone types.
J Biochem Biophys Methods. 1996 Feb 5;31(3-4):81-91.

Reproducibility of IPG-Dalt

Blomberg A, Blomberg L, Norbeck J, Fey SJ, Larsen PM, Larsen M, Roepstorff, P, Degand H, Boutry M, Posch A, Görg, A.
Interlaboratory reproducibility of yeast protein patterns analyzed by
immobilized pH gradient two-dimensional gel electrophoresis.
Electrophoresis. 1995 Oct;16(10):1935-45.

Corbett JM, Dunn MJ, Posch A, Görg A.
Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: an interlaboratory comparison.
Electrophoresis. 1994 Aug-Sep;15(8-9):1205-11.

Sample Prefractionation for Proteome Analysis

Görg A, Boguth G, Kopf A, Reil G, Parlar H, Weiss W.
Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels.
Proteomics. 2002 Dec;2(12):1652-7.

Harder A, Wildgruber R, Nawrocki A, Fey SJ, Larsen PM, Görg A. Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis.
Electrophoresis. 1999 Apr-May;20(4-5):826-9.

Weiss W, Postel W, Görg A.
Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2-D polypeptide patterns of barley seed proteins.
Electrophoresis. 1992 Sep-Oct;13(9-10):770-3.

MALDI - MS

Wildgruber R, Reil G, Drews O, Parlar H, Görg A.
Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Proteomics. 2002 Jun;2(6):727-32.

Mortz E, Krogh TN, Vorum H, Görg A.
Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis.
Proteomics. 2001 Nov;1(11):1359-63.

Nawrocki A, Larsen MR, Podtelejnikov AV, Jensen ON, Mann M, Roepstorff P, Görg A, Fey SJ, Larsen PM.
Correlation of acidic and basic carrier ampholyte and immobilized pH gradient two-dimensional gel electrophoresis patterns based on mass spectrometric protein identification.
Electrophoresis. 1998 May;19(6):1024-35.

Methods

Görg A, Weiss W.
Horizontal SDS-PAGE for IPG-Dalt.
Methods Mol Biol. 1999;112:235-44.

Görg A, Weiss W.
Analytical IPG-Dalt.
Methods Mol Biol. 1999;112:189-95.

Görg A, Boguth G, Obermaier C, Posch A, Weiss W.
Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems.
Electrophoresis. 1995 Jul;16(7):1079-86.

Görg A.
Two-dimensional electrophoresis with immobilized pH gradients.
Biochem Soc Trans. 1993 Feb;21(1):130-2.

Görg A, Postel W, Friedrich C, Kuick R, Strahler JR, Hanash SM. Temperature-dependent spot positional variability in two-dimensional polypeptide patterns.
Electrophoresis. 1991 Sep;12(9):653-8.

Gaveby BM, Pettersson P, Andrasko J, Ineva-Flygare L, Johannesson U, Görg A, Postel W, Domscheit A, Mauri PL, Pietta P, et al.
Stable storage conditions of Immobiline chemicals for isoelectric focusing.
J Biochem Biophys Methods. 1988 Jun-Jul;16(2-3):141-64.

Strahler JR, Hanash SM, Somerlot L, Bjellqvist B, Görg A.
Effect of salt on the performance of immobilized pH gradient isoelectric focusing gels.
Electrophoresis. 1988 Feb;9(2):74-80.

Görg A, Postel W, Gunther S, Friedrich C.
Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem.
Electrophoresis. 1988 Jan;9(1):57-9.


Görg A, Postel W, Gunther S, Weser J, Strahler JR, Hanash SM, Somerlot L, Kuick R.
Approach to stationary two-dimensional pattern: influence of focusing time and immobiline/carrier ampholytes concentrations.
Electrophoresis. 1988 Jan;9(1):37-46.


Hanash, S.M., J.R. Strahler, L. Somerlot, W. Postel and Görg, A:
Two-dimensional electrophoresis with immobilized pH gradients in the first dimension:
Protein focusing as a function of time.
Electrophoresis 1987, 8: 229-234.


Görg A., W. Postel, J. Weser, S. Günther, J.R. Strahler, S.M. Hanash and L. Somerlot:
Horizontal two-dimensional electrophoresis with immobilized pH gradients in the first
dimension in the presence of nonionic detergents
Electrophoresis 1987, 8: 45-51.


Görg A., W. Postel, S. Günther and J. Weser:
Improved horizontal two-dimensional electrophoresis with hybrid isoelectric focusing in
immobilized pH gradients in the first dimension and laying-on transfer to the second
dimension
Electrophoresis 1985, 6: 599-604.


Görg A., Postel W, Westermeier R, Gianazza E, Righetti PG.
Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polyacrylamide layers.
J Biochem Biophys Methods. 1980 Nov;3(5):273-84.


Görg A, Postel W, Westermeier R.
Ultrathin-layer isoelectric focusing in polyacrylamide gels on cellophane.
Anal Biochem. 1978 Aug 15;89(1):60-70.

Proteomic database

Drews O, Görg A
DynaProt 2D: an advanced proteomic database for dynamic online access to proteomes and two-dimensional electrophoresis gels.
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D583-7.

Applications

Arevalo-Ferro C, Reil G, Görg A, Eberl L, Riedel K.
Biofilm formation of Pseudomonas putida IsoF: the role of quorum sensing as assessed by proteomics.
Syst Appl Microbiol. 2005 Mar;28(2):87-114.

Arevalo-Ferro C, Buschmann J, Reil G, Görg A, Wiehlmann L, Tummler B, Eberl L, Riedel K.
Proteome analysis of intraclonal diversity of two Pseudomonas aeruginosa TB clone isolates.
Proteomics. 2004 May;4(5):1241-6.

Arevalo-Ferro, C., Hentzer, M., Reil, G.,Görg A., Kjelleberg, S., Givskov, M., Riedel, K., and Eberl L.
Identification of quorum-sensing regulated proteins in the opportunistic pathogen Pseudomonas aeruginosa by proteomics.
Environ Microbiol. 2003, 5:1350-69

Riedel K, Arevalo-Ferro C, Reil G, Görg A, Lottspeich F, Eberl L.
Analysis of the quorum-sensing regulon of the opportunistic pathogen
Burkholderia cepacia H111 by proteomics.
Electrophoresis. 2003 Feb;24(4):740-50.


Ehling-Schulz M, Schulz S, Wait R, Görg A, Scherer S.
The UV-B stimulon of the terrestrial cyanobacterium Nostoc commune comprises early shock proteins and late acclimation proteins.
Mol Microbiol. 2002 Nov;46(3):827-43.

Drews O, Weiss W, Reil G, Parlar H, Wait R, Görg A.
High pressure effects step-wise altered protein expression in Lactobacillus sanfranciscensis.
Proteomics. 2002 Jun;2(6):765-74.


Scheibe B, Weiss W, Rueff F, Przybilla B, Görg A.
Detection of trace amounts of hidden allergens: hazelnut and almond proteins in chocolate.
J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):229-37.


Regula JT, Boguth G, Görg A, Hegermann J, Mayer F, Frank R, Herrmann R.
Defining the mycoplasma 'cytoskeleton': the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry.
Microbiology. 2001 Apr;147(Pt 4):1045-57.


Scheibe B., Weiss W., Bauer, C.-P., Görg, A.
Electrophoretic and immunochemical determination of bioindustrial enzymes in apple juice.
Eur. Food Res. Technol. 2001, 212: 691-95

Rogowska-Wrezesinska, A., Larsen, P.M., Blomberg, A., Görg, A.., Roepstorff, P., Norbeck, J., and Fey, S.J.
Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303.
Comparative and Functional Genomics 2001, 2: 207-225


Regula JT, Ueberle B, Boguth G, Görg A, Schnolzer M, Herrmann R, Frank R.
Towards a two-dimensional proteome map of Mycoplasma pneumoniae.
Electrophoresis. 2000 Nov;21(17):3765-80.


Neuhaus K, Francis KP, Rapposch S, Görg A, Scherer S.
Pathogenic Yersinia species carry a novel, cold-inducible major cold shock protein tandem gene duplication producing both bicistronic and monocistronic mRNA.
J Bacteriol. 1999 Oct;181(20):6449-55.


Fey SJ, Nawrocki A, Larsen MR, Görg A, Roepstorff P, Skews GN, Williams R, Larsen PM.
Proteome analysis of Saccharomyces cerevisiae: a methodological outline.
Electrophoresis. 1997 Aug;18(8):1361-72.


Weiss W, Huber G, Engel KH, Pethran A, Dunn MJ, Gooley AA, Görg A.
Identification and characterization of wheat grain albumin/globulin allergens.
Electrophoresis. 1997 May;18(5):826-33.


Posch A, van den Berg BM, Burg HC, Görg A.
Genetic variability of carrot seed proteins analyzed by one- and two-dimensional electrophoresis with immobilized pH gradients.
Electrophoresis. 1995 Jul;16(7):1312-6.


Posch A, Weiss W, Wheeler C, Dunn MJ, Görg A.
Sequence analysis of wheat grain allergens separated by two-dimensional electrophoresis with immobilized pH gradients.
Electrophoresis. 1995 Jul;16(7):1115-9.


Posch A, van den Berg BM, Görg A.
Association of protein polymorphism among pepper (Capsicum annuum L.) inbred lines with agronomic performance of their crosses.
Electrophoresis. 1995 May;16(5):860-4.


Posch A, van den Berg BM, Duranton C, Görg A.
Polymorphism of pepper (Capsicum annuum L.) seed proteins studied by two-dimensional electrophoresis with immobilized pH gradients: methodical and genetic aspects.
Electrophoresis. 1994 Feb;15(2):297-304.


Weiss W, Vogelmeier C, Görg A.
Electrophoretic characterization of wheat grain allergens from different cultivars involved in bakers' asthma.
Electrophoresis. 1993 Aug;14(8):805-16.


Brodard V, David B, Görg A, Peltre G.
Two-dimensional gel electrophoretic analysis with immobilized pH gradients of Dactylis glomerata pollen allergens.
Int Arch Allergy Immunol. 1993;102(1):72-80.


Weiss W, Postel W, Görg A.
Qualitative and quantitative changes in barley seed protein patterns during the malting process analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with respect to malting quality.
Electrophoresis. 1992 Sep-Oct;13(9-10):787-97.


Posch A, van den Berg BM, Postel W, Görg A.
Genetic variability of pepper (Capsicum annuum L.) seed proteins studied by 2-D electrophoresis with immobilized pH gradients.
Electrophoresis. 1992 Sep-Oct;13(9-10):774-7.


Görg A, Postel W, Weiss W.
Detection of polypeptides and amylase isoenzyme modifications related to malting quality during malting process of barley by two-dimensional electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis. 1992 Sep-Oct;13(9-10):759-70.


Görg A, Postel W, Baumer M, Weiss W.
Two-dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: discrimination of cultivars with different malting grades.
Electrophoresis. 1992 Apr;13(4):192-203.


Easty D, Hart IR, Patel K, Seymour C, Yacoub M, Domscheit A, Gunther S, Postel W, Görg A, Dunn MJ.
Changes in protein expression during melanoma differentiation determined by computer analysis of 2-D gels.
Clin Exp Metastasis. 1991 May-Jun;9(3):221-30.


Weiss W, Postel W, Görg A.
Barley cultivar discrimination: II. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing with immobilized pH gradients.
Electrophoresis. 1991 May;12(5):330-7.


Weiss W, Postel W, Görg A.
Barley cultivar discrimination: I. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and glycoprotein blotting.
Electrophoresis. 1991 May;12(5):323-30.


Zastrow G, Gunther S, Postel W, Görg A, Diehl HA, Jansen EH.
Serum proteins of rats exposed to organic solvents examined by horizontal two-dimensional electrophoresis with an immobilized pH gradient in the first dimension.
Electrophoresis. 1990 Aug;11(8):655-7.


Görg A, Postel W, Domscheit A, Gunther S.
Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): method, reproducibility and genetic aspects.
Electrophoresis. 1988 Nov;9(11):681-92.


Gunther S, Postel W, Wiering H, Görg A.
Acid phosphatase typing for breeding nematode-resistant tomatoes by isoelectric focusing with an ultranarrow immobilized pH gradient.
Electrophoresis. 1988 Sep;9(9):618-20.


Patel K, Dunn MJ, Gunther S, Postel W, Görg A.
Dual-label autoradiographic analysis of human skin fibroblast and myoblast proteins by two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradients in the first dimension.
Electrophoresis. 1988 Sep;9(9):547-54.


Strahler, J.R., S.M. Hanash, L. Somerlot, J. Weser, W. Postel and Görg, A:
High resolution two-dimensional polyacrylamide gel electrophoresis of basic myeloid
polypeptides: Use of immobilized pH gradients in the first dimension.
Electrophoresis 1987, 8: 165-173.


Schiwara, H., T. Hebell, H. Kirchherr, W. Postel, J. Weser and A. Görg:
Ultrathin-layer sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis and
silver staining of urinary proteins
Electrophoresis 1986, 7: 496-505.


Görg A, Postel W, Weser J, Patutschnick W, Cleve H.
Improved resolution of PI (alpha 1-antitrypsin) phenotypes by a large-scale immobilized pH gradient.
Am J Hum Genet. 1985 Sep;37(5):922-30.


Görg A
, Postel W, Johann P.
pH, urea and substrate gradients for the optimization of ultrathin polyacrylamide gel zymograms.
J Biochem Biophys Methods. 1985 Mar;10(5-6):341-50.

Westermeier, R., W. Postel, A. Görg and L. Telek:
Isoelectric focusing and sodium dodecyl sulphate electrophoresis of buffer soluble seed
proteins of twelve varieties of winged bean (Psophocarpus tetragonolobus L.)
J. Sci. Food Agric. 1985, 36: 135-141.


Weidinger S, Cleve H, Schwarzfischer F, Postel W, Weser J, Görg A.
Transferrin subtypes and variants in Germany; further evidence for a Tf null allele.
Hum Genet. 1984;66(4):356-60.


Westermeier R, Postel W, Weser J, Görg A.
High-resolution two-dimensional electrophoresis with isoelectric focusing in immobilized pH gradients.
J Biochem Biophys Methods. 1983 Dec;8(4):321-30.


Görg A, Weser J, Westermeier R, Postel W, Weidinger S, Patutschnick W, Cleve H.
Isoelectric focusing with immobilized pH gradients for the analysis of transferrin (Tf) subtypes and variants.
Hum Genet. 1983;64(3):222-6.


Görg, A., W. Postel, J. Weser, S. Weidinger, W. Patutschnick and H. Cleve:
Isoelectric focusing in immobilized pH gradients for the determination of the genetic Pi (?1-
antitrypsin) variants
Electrophoresis 1983, 4: 153-157.

Cleve, H., W. Patutschnick, W. Postel, J. Weser and A. Görg:
Analysis of the genetic variants of the human Gc system (VDBP) by isoelectric focusing in
immobilized pH gradients
Electrophoresis 1982, 3: 342-345.


Book Chapters

Westermeier, R., and Görg, A.
Two-dimensional electrophoresis in proteomics.
In: J.C. Janson (Ed.)., Protein Purification, 3rd edition, Wiley & Sons, New York, 2006 (in press)

Westermeier, R., and Görg, A.
Elektrophoretische Verfahren.
In: Lottspeich (Ed.)., Bioanalytik, 2nd edition, Elsevier, Heidelberg, 2006, pp. 235-268

Weiss, W., and Görg, A.
Two dimensional electrophoresis for plant proteomics.
In: Methods in Molecular Biology: Plant Proteomics -Method and Protocols (H. Thiellement et al., Eds.), Ch. 13, Humana, NJ, 2006, pp. 121-143

Görg, A., and Weiss, W.
High resolution Two-dimensional Electrophoresis with Immobilized pH gradients for Proteome Analysis.
In: Cell Biology. A Laboratory Manual, Vol. IV, Ch. 23, 3rd edition. (J.E. Celis, Ed.), Elsevier Science & Academic Press, 2006, pp. 175-188

Görg, A., Weiss, W., and Dunn, MJ.
Two-dimensional gel electrophoresis.
In: M.J.Dunn (Ed.), Encyclopaedia of Genomics, Proteomics and Bioinformatics. Vol. 5. Wiley & Sons, 2005, pp. 1903-1915

Görg, A.., Drews, O., and Weiss, W.
Separation of proteins using two-dimensional gel electrophoresis.
In: R.J. Simpson (Ed.), Purifying proteins for Proteomics: A Laboratory Manual.
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2004, pp. 391-430

Görg, A.., and Weiss, W.
Proteome profile comparisons of microorganisms, cells & tissues using 2-D gels.
In: Proteome Analysis: Interpreting the genome (D.W. Speicher, Ed.). Elsevier, 2004, pp. 19-73


Dunn, M.J, Görg, A.:
Two-dimensional polyacrylamide gel electrophoresis for proteome analysis.
In: Proteomics (Pennington, S, Dunn, M.J., eds.). BIOS Scientific Publishers, Oxford 2000
pp 43-63

Görg, A.:
Advances in 2D gel techniques.
Proteomics: A Trends Guide. Elsevier Science, London, 2000 pp. 3-6


Görg, A., Weiss, W.:
2D electrophoresis with immobilized pH gradients. In: Proteome Research: Two-Dimensional
Electrophoresis and Identification Methods. Springer, Berlin Heidelberg New York, 2000,
57-106 (2000)

Görg, A., Westermeier, R.:
High Resolution Gel-Electrophoretic Techniques: Qualitative, Quantitative and
Micropreparative Applications. In: Microcharacterization of Proteins. R. Kellner, F.
Lottspeich, H.E. Meyer (Eds.). VCH, Weinheim 1999, pp 13-24


Görg, A., Westermeier, R.:
Elektrophoretische Trennverfahren. In: Bioanalytik. F. Lottspeich und H. Zorbas (Hrsg.).
Spektrum Akademischer Verlag, Heidelberg Berlin 1998, 217-252


Westermeier, R., Görg, A.:
Protein mapping by two-dimensional polyacrylamide gel electrophoresis. In: Protein
Purification. Principles, High-Resolution Methods and Applications.
J.-C. Janson and L. Ryden (Eds.). 2nd edition. Wiley-VCH, New York 1998, pp. 557-586


Görg, A., Weiss, W.:
High resolution two-dimensional electrophoresis of proteins using immobilized pH gradients.
In: J. Celis (Ed.), Cell Biology: A Laboratory Handbook, 2nd edition Vol. 4, Academic Press,
San Diego, CA, 1998, pp 386-397


Görg, A., J.S. Fawcett, A. Chrambach:
The current state of electrofocusing in immobilized pH gradients
Advances in Electrophoresis (A. Chrambach, M.J. Dunn, B.J. Radola, Eds.), VCH-Verlag,
Weinheim 1988, pp 1-44.