PD. Dr. Karl Kramer

Position:	Group Leader Cell Biology
Room:	005
Category:	Scientific Staff
Phone:	+49 - (0)8161 - 714477
Email:

Publications

Bioassays for estrogenic and androgenic effects of water constituents.

Treatise on Water Science. 2011 Jan; Vol. 3:191-219. ISBN: 978-0-444-53193-3

Kramer K

Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer.

Mol Cell Proteomics. 2011 Sep 28. [Epub ahead of print] DOI: -

Online reference

An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits.

J AOAC Int. 2010 Jan-Feb;93(1):80-8. DOI: -

Online reference

Development of a monoclonal antibody for the detection of a potentially allergenic epitope in soy.

Anal Lett. 2010 Jan; 43(1):119-32. DOI: DOI: 10.1080/00032710903276521

Mahlknecht G | Hock B | Kramer K

A system for repertoire cloning and phage display of murine and leporid antibody fragments.

J AOAC Int. 2010 Jan-Feb;93(1):66-79. DOI: -

Online reference

Molecular antibody technologies for biosensors and bioanalytics.

Handbook of Biosensors and Biochips. 2008 Mar; 101-113. ISBN: 10.1002/9780470061565.hbb007

Kramer K | Mahlknecht G | Hock B

Production of antibodies for the quantitative detection of the anabolically active androgens 17β-boldenone and methylboldenone.

Anal Lett. 2007 Jan; 40(7):1461-72. DOI: 10.1080/00032710701327112

Kramer K | Hubauer A | Lausterer R | Marco M-P | Salvador J-P

Directed evolution of ligand-binding proteins.

Immunoassay and Other Bioanalytical Techniques. 2007 Jan; 45–74. ISBN: 10.1201/9781420020694.ch2

Kramer K | Geue H | Hock B

Recombinant Antibodies for Agrochemicals: Evolutionary Optimization

Rational Environmental Management of Agrochemicals 2007 Sep; 155-170. ISBN: 9780841274204

Kramer K | Hock B

Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) of the pyrethroid bioallethrin in food and environmental samples.

J Agric Food Chem. 2006 Sep 6;54(18):6482-92. DOI: -

Online reference

Analyte peptidomimetics selected from phage display peptide libraries: a systematic strategy for the development of environmental immunoassays.

Environ Sci Technol. 2005 Jun 1;39(11):4234-41. DOI: -

Online reference

Recombinant antibodies for environmental analysis.

Anal Bioanal Chem. 2003 Oct;377(3):417-26. Epub 2003 Sep 2. DOI: -

Kramer K | Hock B

Online reference

Comparison of affinity ranking and immunochemical key data as measure for molecular antibody evolution.

Spectroscopy 2003 Jan; 17(2-3):355-65. DOI:

Kramer K | Rau D | Hock B

Engineering receptors and antibodies for biosensors.

Biosens Bioelectron. 2002 Mar;17(3):239-49. DOI: -

Hock B | Seifert M | Kramer K

Online reference

Synthesis of a group-selective antibody library against haptens.

J Immunol Methods. 2002 Aug 1;266(1-2):209-20. DOI: -

Kramer K

Online reference

A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability.

Biosens Bioelectron. 2002 Apr;17(4):305-13. DOI: -

Kramer K | Fiedler M | Skerra A | Hock B

Online reference

Development of ELISA technique for the analysis of atrazine residues in water.

J Environ Sci Health B. 2002 Jul;37(4):307-22. DOI: -

Online reference

C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates.

Mol Microbiol. 2002 Apr;44(2):335-49. DOI: -

Loessner MJ | Kramer K | Ebel F | Scherer S

Online reference

Evolutionary affinity and selectivity optimization of a pesticide-selective antibody utlizing a hapten-selective immunoglobulin repertoire.

Environ Sci Technol. 2002 Nov 15;36(22):4892-8. DOI: -

Kramer K

Online reference

Environmental analysis using antibody and receptor based techniques.

Int J Environ An Ch. 2001 Nov; 78(3-4):289-303. DOI: 10.1080/03067310008041348

Hock B | Kramer K | Seifert M

Long-term monitoring of atrazine contamination in soil by ELISA.

J AOAC Int. 2001 Jan-Feb;84(1):150-5. DOI: -

Kramer K | Lepschy J | Hock B

Online reference

Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes.

Anal Bioanal Chem. 2002 Jan;372(2):261-7. Epub 2001 Dec 22. DOI: -

Rau D | Kramer K | Hock B

Online reference

Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein.

J Clin Microbiol. 2000 Jul;38(7):2643-8. DOI: -

Online reference

Stabilisation of immunoassays and receptor assays.

J Mol Catal B-Enzym. 1999 Sep; 7(1):115-124. DOI: 10.1016/S1381-1177(99)00036-3

Synthesis of pesticide-specific single-chain Fv utilizing the Recombinant Phage Antibody System (RPAS, Pharmacia). A detailed protocol.

Anal Lett. 1998 Jan; 31(1):67-92. DOI: 10.1080/00032719808001834

Kramer K

Affinity characterization of monoclonal and recombinant antibodies for multianalyte detection with an optical transducer.

Sensor Actuat B-Chem. 1997 Mar; 39(1-3):432-7. DOI: 10.1016/S0925-4005(96)02118-1

Non-extractable pesticide residues in humic substances: immunochemical analysis.

Immunochemical Technology for Environmental Applications. 1997 May; 290-302. ISBN: 10.1021/bk-1997-0657.ch024

Recombinant antibodies for pesticide immunoanalysis.

Immunoassays for Residue Analysis. 1996 May; 471-484. ISBN: 10.1021/bk-1996-0621.ch038

Kramer K | Hock B

Recombinant single-chain antibodies against s-triazines.

Food Agr Immunol. 1996 Jun; 8(2):97-109. DOI: 10.1080/09540109609354908

Kramer K | Hock B

Multi-analyte determination with a direct optical multi-antibody detection system.

Environmental Monitoring and Hazardous Waste Site Remediation. 1995 Jun; 185-193. ISBN: 0819418625

Piehler J | Brecht A | Kramer K | Hock B | Gauglitz G

Immunochemical techniques: antibody production for pesticide analysis. A review.

Anal Chim Acta 1995 Aug; 311(3):393-405. DOI: 10.1016/0003-2670(95)00148-S

Hock B | Dankwardt A | Kramer K | Marx A

Atrazine residues in soil two years after the atrazine ban - A comparison of enzyme immunoassay with HPLC.

Anal Lett. 1995 Apr; 28(4):621-634. DOI: 10.1080/00032719508001122

Magnetic bead selection of hybridomas producing pesticide antibodies.

Food Agr Immunol. 1994 March; 6(1):5-16. DOI: 10.1080/09540109409354807

Kramer K | Giersch T | Hock B

Toxicity assessment and on-line monitoring: Immunoassays.

Environ Toxic Water. 1994 Nov; 9(4):243–62. DOI: 10.1002/tox.2530090402

Hock B | Giersch T | Dankwardt A | Kramer K | Pullen S

Monoclonal antibody-based enzyme immunoassays for the sensitive detection of s-triazines in water.

SPIE 1993 Mar; 1716:26-36. DOI: 10.1117/12.140273

Hock B | Giersch T | Kramer K

Optimization of a monoclonal antibody-based enzyme immunoassay for the detection of terbuthylazine.

Sci Total Environ. 1993 Apr 29;132(2-3):435-48. DOI: -

Giersch T | Kramer K | Hock B

Online reference

Improvement of a monoclonal antibody-based immunoassay for the determination of terbutryn.

Acta hydrochim hydrobiol. 1993; 21(6):312-5. ISBN: 10.1002/aheh.19930210604

Giersch T | Kramer K | Weller M | Hock B

Immunoassays for environmental analysis.

Analusis. 1992; 20(8):29-33. DOI:

Hock B | Giersch T | Kramer K

Antibody production and progress in hybridoma technology by immunomagnetic screening.

New Frontiers in Agrochemical Immunoassay. 1995; 149-162. ISBN: 0-935584-58-7

Hock B | Giersch T | Kramer K | Dankwardt A

Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA.

J Immunoassay Immunochem. 2002;23(2):129-43. DOI: -

Rau D | Kramer K | Hock B

Online reference

Molecular basis of toxic effects: Inhibition of cellular pathways and structural components.

Plant toxicology. 2005; 331-468. ISBN: 0824753232

Kramer K | Hock B

Antibodies for biosensors.

Ultrathin Electrochemical Chemo- and Biosensors. 2004; 3-22. ISBN: 978-3-540-21285-0

Kramer K | Hock B

Strategies for recombinant antibody library synthesis: An advanced source for immunoglobulins in environmental analysis.

Biosensors for Environmental Diagnostics. 1998; 161-178. ISBN: 3-8154-3540-4

Kramer K | Knappik A

Production and application of monoclonal antibodies for the determination of pesticides.

Immunochemical Detection of Pesticides and Their Metabolites in the Water Cycle. 1995; 25-42. ISBN: 3527271376

Giersch T | Kramer K

back

PD. Dr. Karl Kramer

Publications

Bioassays for estrogenic and androgenic effects of water constituents.

Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer.

An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits.

Development of a monoclonal antibody for the detection of a potentially allergenic epitope in soy.

A system for repertoire cloning and phage display of murine and leporid antibody fragments.

Molecular antibody technologies for biosensors and bioanalytics.

Production of antibodies for the quantitative detection of the anabolically active androgens 17β-boldenone and methylboldenone.

Directed evolution of ligand-binding proteins.

Recombinant Antibodies for Agrochemicals: Evolutionary Optimization

Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) of the pyrethroid bioallethrin in food and environmental samples.

Analyte peptidomimetics selected from phage display peptide libraries: a systematic strategy for the development of environmental immunoassays.

Recombinant antibodies for environmental analysis.

Comparison of affinity ranking and immunochemical key data as measure for molecular antibody evolution.

Engineering receptors and antibodies for biosensors.

Synthesis of a group-selective antibody library against haptens.

A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability.

Development of ELISA technique for the analysis of atrazine residues in water.

C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates.

Evolutionary affinity and selectivity optimization of a pesticide-selective antibody utlizing a hapten-selective immunoglobulin repertoire.

Environmental analysis using antibody and receptor based techniques.

Long-term monitoring of atrazine contamination in soil by ELISA.

Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes.

Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein.

Stabilisation of immunoassays and receptor assays.

Synthesis of pesticide-specific single-chain Fv utilizing the Recombinant Phage Antibody System (RPAS, Pharmacia). A detailed protocol.

Affinity characterization of monoclonal and recombinant antibodies for multianalyte detection with an optical transducer.

Non-extractable pesticide residues in humic substances: immunochemical analysis.

Recombinant antibodies for pesticide immunoanalysis.

Recombinant single-chain antibodies against s-triazines.

Multi-analyte determination with a direct optical multi-antibody detection system.

Immunochemical techniques: antibody production for pesticide analysis. A review.

Atrazine residues in soil two years after the atrazine ban - A comparison of enzyme immunoassay with HPLC.

Magnetic bead selection of hybridomas producing pesticide antibodies.

Toxicity assessment and on-line monitoring: Immunoassays.

Monoclonal antibody-based enzyme immunoassays for the sensitive detection of s-triazines in water.

Optimization of a monoclonal antibody-based enzyme immunoassay for the detection of terbuthylazine.

Improvement of a monoclonal antibody-based immunoassay for the determination of terbutryn.

Immunoassays for environmental analysis.

Antibody production and progress in hybridoma technology by immunomagnetic screening.

Single-chain Fv antibody-alkaline phosphatase fusion proteins produced by one-step cloning as rapid detection tools for ELISA.

Molecular basis of toxic effects: Inhibition of cellular pathways and structural components.

Antibodies for biosensors.

Strategies for recombinant antibody library synthesis: An advanced source for immunoglobulins in environmental analysis.

Production and application of monoclonal antibodies for the determination of pesticides.

Chair of Proteomics and Bioanalytics

NEWS

13 October 2011

25 April 2011

29 March 2011

26 November 2010

09 November 2010